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KMID : 1143420210140080421
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Public Health Weekly Report
2021 Volume.14 No. 8 p.421 ~ p.428
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Review of Laboratory-based Diagnosis of Hemorrhagic Fever with Renal Syndrome and Considerations for Improving Antibody Tests
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Lim A-Ram
Kang Hae-Ji
Han Myung-Guk
Oh Sea-Jin
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Abstract
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Hemorrhagic fever with renal syndrome (HFRS) is a feverish disease caused by hantavirus infection that occurs mainly in
autumn in Korea. The primary aim of this review was to analyze the 2017-2019 results of laboratory tests for HFRS to find
improvements in the present antibody diagnostic method. HFRS antibody diagnostics is required both acute and
convalescent phase serum to confirm an increase in antibody titer. In the last 3 years, 86.3% of HFRS tests were requested
only for acute phase serum, but not for convalsecent phase serum. Since most of the primary single serum samples were
submitted to laboratory, a 98.4% level of test result was pending. To address limitations of the IFA test, we developed the
ELISA. The IgM ELISA for antibody test of HFRS was expected to provide rapid and sensitive results by detecting IgM
antibodies from single serum samples in the acute phase. Furthermore, the results of the HFRS IgG avidity test-based ELISA could provide scientific evidence for the 1:512 standard of antibody titer of IFA test. The developed ELISA kit will also be used by Public Health and Environmental Research Institutes (PHERIs) will be effectively used in the diagnosis of HFRS in the future.
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KEYWORD
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Hemorrhagic fever with renal syndrome (HFRS), Hantaan virus, Indirect Immuno-fluorescence Antibody Assay (IFA), Enzyme-Linked Immunosorbent Assay (ELISA)
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